Original Article
Volume: 41 | Issue: 1 | Published: Mar 25, 2025 | Pages: 08 - 08 | DOI: 10.24911/BioMedica/5-1209
Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore
Authors:
Aneela qaisar
, Ayesha Irfan
, Aribah Nawaz Chatta
, Azree Sohail
, Zuhaa Waqar Azim
, Muneeba Saleem Malik
, Mahnoor Nasr
, Alia Batool
Article Info
Authors
Aribah Nawaz Chatta
Fatima Memorial Hospital College of Medicine and Dentistry (FMHCMD)
Azree Sohail
Fatima Memorial Hospital College of Medicine and Dentistry (FMHCMD)
Zuhaa Waqar Azim
Fatima Memorial Hospital College of Medicine and Dentistry (FMHCMD)
Muneeba Saleem Malik
Fatima Memorial Hospital College of Medicine and Dentistry (FMHCMD)
Mahnoor Nasr
Fatima Memorial Hospital College of Medicine and Dentistry (FMHCMD)
Alia Batool
Fatima Memorial Hospital College of Medicine and Dentistry (FMHCMD)
Publication History
Received: September 22, 2024
Accepted: January 06, 2025
Published: March 25, 2025
Abstract
Background and Objective: Dental laboratory surfaces are contaminated by various microorganisms that can cause infections among staff and students. This observational study aimed to identify different types of microbial flora present on different surfaces of dental laboratories and determine the antibiotic sensitivity of the isolates.
Methods: This cross-sectional observational study was conducted in the dental laboratory of a dental school in Pakistan from April 2023 to April 2024. Eleven samples were collected from randomly selected surface areas in the dental laboratory during working hours of the day without any prior disinfection using Amies agar gel transport swabs and transferred to the laboratory for culture and sensitivity testing. Visible growth was observed on all culture plates. Colonies grown on blood and MacConkey agar plates were tested using standard microbiological methods.
Results: Analysis of swabs taken from the dental laboratory surfaces showed microbial contamination with Acinetobacter baumannii (63%), Klebsiella pneumoniae (36%), and Staphylococcus hominis (27%), with the absence of any fungal growth. These microorganisms showed variable resistance to various antibiotics, including Ampicillin, Co-amoxiclav, Co-trimoxazole, Meropenem, Ciprofloxacin, Cefotaxamine, and Levofloxacin.
Conclusion: This study found pathogenic microorganisms resistant to most antibiotics, highlighting the need to update disinfection practices commonly used in our dental laboratories.
Keywords: Acinetobacter baumannii, antibiotic, antimicrobial resistance, dental laboratory.
Pubmed Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool. Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore. BioMedica. 2025; 25 (March 2025): 08-08. doi:10.24911/BioMedica/5-1209
Web Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool. Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore. https://biomedicapk.com/index.php/articles/1209 [Access: August 10, 2025]. doi:10.24911/BioMedica/5-1209
AMA (American Medical Association) Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool. Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore. BioMedica. 2025; 25 (March 2025): 08-08. doi:10.24911/BioMedica/5-1209
Vancouver/ICMJE Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool. Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore. BioMedica. (2025), [cited August 10, 2025]; 25 (March 2025): 08-08. doi:10.24911/BioMedica/5-1209
Harvard Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool (2025) Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore. BioMedica, 25 (March 2025): 08-08. doi:10.24911/BioMedica/5-1209
Chicago Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool. "Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore." 25 (2025), 08-08. doi:10.24911/BioMedica/5-1209
MLA (The Modern Language Association) Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool. "Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore." 25.March 2025 (2025), 08-08. Print. doi:10.24911/BioMedica/5-1209
APA (American Psychological Association) Style
Aneela qaisar, Ayesha Irfan, Aribah Nawaz Chatta, Azree Sohail, Zuhaa Waqar Azim, Muneeba Saleem Malik, Mahnoor Nasr, Alia Batool (2025) Spectrum of antibiotic sensitivity of bacterial flora isolated from dental laboratory surfaces at a private dental hospital in Lahore. , 25 (March 2025), 08-08. doi:10.24911/BioMedica/5-1209