Original Article

VOLUME: 36 | ISSUE: 3 | Sep 30, 2020 | PAGE: (278 - 284) | DOI: 10.24911/BioMedica/5-108

Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment


Authors: Lizhen Xu , Shanshan Gao , Jingjing Jiang , Mei Sun , Yan Sheng , Rong Tang ,


Authors

Lizhen Xu

Department of Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai – China.

Shanshan Gao

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan – China.

Jingjing Jiang

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan – China.

Mei Sun

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan – China.

Yan Sheng

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan – China.

Rong Tang

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan – China.

Publication History

Received: July 10, 2020

Revised: August 19, 2020

Accepted: September 24, 2020

Published: September 30, 2020


Abstract


Background and Objective: Advances in cell media have led to embryo transfer from cleavage to blastocyst. The extension of embryo culture to blastocyst stage provides some theoretical advantages and disadvantages that have been controversial. The objective of this study was to evaluate the clinical efficacy of vitrified warm cutting and blastocyst transplantation in Assisted Reproductive Technique (ART) therapy. 
Methods: The study was performed on 2740 women undergoing frozen embryo thawing transfer. Patients' basic clinical information, status of frozen embryo transfer cycle, clinical pregnancy rate, early abortion rate, sex ratio of birth and birth weight were retrospectively analyzed. The main clinical outcomes of the recovery of frozen embryos at cleavage and blastocyst stages were compared. In addition, the clinical outcomes of blastocyst cryopreservation on the 5th, 6th or 7th day after oocyte retrieval according to the date of blastocyst expansion were recorded.
Results: The implantation ratio of cleavage stage embryos was 21.62% compared with 43.52% on D5 (P <0.05). The D5, D6, and D7 implanting rates were statistically different. The pregnancy rates were 57.56%, 51.76% and 35.95% versus 37.79%, and the early abortion was 23.08%, 15.42% and 22.35% versus 34.55% respectively for embryos cryopreserved on D5, D6, D7 and D3. There were significant differences between D5 and D3 rates of ectopic pregnancy and early abortion. The sex ratio, the birth weight and birth defect were not statistically different among four groups.
Conclusion: Blastocyst transfer achieved a higher implantation rate than vitrified cleavage stage embryo and decreased ectopic pregnancy rate. With increased incubation days before expansion blastocyst formed, the implantation rate is reduced and the early abortion rate increases.


Keywords: Vitrified-warmed, Cleavage stage, Blastocyst, Clinical outcome, Assisted Reproductive Technique


Pubmed Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang. Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment. BioMedica. 2020; 30 (September 2020): 278-284. doi:10.24911/BioMedica/5-108

Web Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang. Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment. https://biomedicapk.com/articles/online_first/108 [Access: May 27, 2024]. doi:10.24911/BioMedica/5-108

AMA (American Medical Association) Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang. Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment. BioMedica. 2020; 30 (September 2020): 278-284. doi:10.24911/BioMedica/5-108

Vancouver/ICMJE Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang. Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment. BioMedica. (2020), [cited May 27, 2024]; 30 (September 2020): 278-284. doi:10.24911/BioMedica/5-108

Harvard Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang (2020) Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment. BioMedica, 30 (September 2020): 278-284. doi:10.24911/BioMedica/5-108

Chicago Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang. "Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment." 30 (2020), 278-284. doi:10.24911/BioMedica/5-108

MLA (The Modern Language Association) Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang. "Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment." 30.September 2020 (2020), 278-284. Print. doi:10.24911/BioMedica/5-108

APA (American Psychological Association) Style

Lizhen Xu, Shanshan Gao, Jingjing Jiang, Mei Sun, Yan Sheng, Rong Tang (2020) Evaluation of the Clinical Efficacy of Vitrification, Warming and Blastocyst Transplantation in Assisted Reproductive Treatment. , 30 (September 2020), 278-284. doi:10.24911/BioMedica/5-108