Original Article

VOLUME: 36 | ISSUE: 3 | Sep 30, 2020 | PAGE: (285 - 290) | DOI: 10.24911/BioMedica/5-112

Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial


Authors: Ghazala Hassan , Sarah Ghafoor , Saima Chaudhry , Zubair Ahmed Khan


Authors

Ghazala Hassan

Department of Oral Biology, University of Health Sciences, Lahore-Pakistan.

Sarah Ghafoor

Department of Oral Biology, University of Health Sciences, Lahore-Pakistan.

Saima Chaudhry

Department of Oral Pathology, University of Health Sciences, Lahore-Pakistan.

Zubair Ahmed Khan

Department of Periodontology, Fatima Memorial College of Medicine and Dentistry, Lahore-Pakistan..

Publication History

Received: July 06, 2020

Revised: August 02, 2020

Accepted: September 24, 2020

Published: September 30, 2020


Abstract


Background and Objective: Salivary level of Matrix metalloproteinase-8 (MMP-8), a collagen degradation enzyme, is known to increase during chronic periodontitis. Nigella sativa is one of the potent anti[1]inflammatory compounds that has a promising role in oral inflammatory conditions. Present study aims to explore the salivary MMP-8 levels after Nigella sativa oil-based mouth wash as compared to placebo(normal saline) after non-surgical periodontal therapy in patients with chronic periodontitis.

Methods: This parallel arm randomized controlled trial was conducted at FMH College of Medicine & Dentistry, Lahore Pakistan from January 2018 to June 2019. Fifty patients suffering from chronic periodontitis were recruited and randomized into the treatment group that received Nigella sativa oil and the control group that received simple normal saline. Salivary samples for MMP-8 levels were collected , once before non-surgical periodontal therapy and secondly after two weeks of rinsing with Nigella sativa oil-based mouthwash in one group and normal saline in the second. Salivary MMP-8 levels were determined through ELISA. The comparison within groups was done through paired t test and between groups through independent sample t-test.

Results: Mean pre-treatment concentration of salivary MMP-8 of the Nigella Sativa group and normal saline group were 199.37 ± 300.75 ng/l and 195.29 ± 175.89 ng/l that decreased post-treatment to 147.35 ± 144.9ng/l and 114.17 ± 129.21ng/l respectively. Statistical analysis revealed no difference in the salivary MMP-8 levels between the chronic periodontitis patients using Nigella sativa or normal saline mouth wash twice daily for two weeks.

Conclusion: The salivary levels of MMP-8 reduced in patients with chronic periodontitis after scaling and root planning with the use of Nigella sativa oil-based mouthwash as well as placebo (normal saline). However, this reduction was statistically non-significant both within and between the study groups.


Keywords: Nigella sativa, Chronic periodontitis, Matrix metalloproteinase-8, Saliva


Pubmed Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan. Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial. BioMedica. 2020; 30 (September 2020): 285-290. doi:10.24911/BioMedica/5-112

Web Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan. Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial. https://biomedicapk.com/articles/online_first/112 [Access: May 27, 2024]. doi:10.24911/BioMedica/5-112

AMA (American Medical Association) Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan. Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial. BioMedica. 2020; 30 (September 2020): 285-290. doi:10.24911/BioMedica/5-112

Vancouver/ICMJE Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan. Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial. BioMedica. (2020), [cited May 27, 2024]; 30 (September 2020): 285-290. doi:10.24911/BioMedica/5-112

Harvard Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan (2020) Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial. BioMedica, 30 (September 2020): 285-290. doi:10.24911/BioMedica/5-112

Chicago Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan. "Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial." 30 (2020), 285-290. doi:10.24911/BioMedica/5-112

MLA (The Modern Language Association) Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan. "Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial." 30.September 2020 (2020), 285-290. Print. doi:10.24911/BioMedica/5-112

APA (American Psychological Association) Style

Ghazala Hassan, Sarah Ghafoor, Saima Chaudhry, Zubair Ahmed Khan (2020) Effect of Nigella Sattiva (Kalonji) Oil Based Mouth Wash on Salivary Matrix Metalloproteinase-8 Level in Patients with Chronic Periodonititis- A Randomized controlled Trial. , 30 (September 2020), 285-290. doi:10.24911/BioMedica/5-112