Original Article
VOLUME: 40 | ISSUE: 2 | Jun 25, 2024 | PAGE: (95 - 99) | DOI: 10.24911/BioMedica/5-1153
Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas
Authors: Hamna Salahuddin , Sadia Sharif , Shizra Kaleemi , Kanwal Babar , Abeer Zaheer , Sadia Anwar
Article Info
Authors
Hamna Salahuddin
University College of Medicine and Dentistry, Ameer-ud-din Medical College
Sadia Sharif
University College of Medicine and Dentistry
Shizra Kaleemi
Al Aleem Medical College
Kanwal Babar
Chughtai Lab, Lahore, Pakistan
Abeer Zaheer
Rahbar Medical & Dental College
Sadia Anwar
Ameeruddin Medical College
Publication History
Received: April 01, 2024
Revised: June 07, 2024
Accepted: June 20, 2024
Published: June 25, 2024
Abstract
Background and Objective: In surgical procedures, accurate intraoperative diagnosis is essential for guiding surgical management. Two common techniques employed for rapid intraoperative diagnosis are touch imprint cytology (TIC) and frozen section (FS). However, their diagnostic accuracy as compared to histopathology remains under debate. This study therefore aims to evaluate the diagnostic precision of FSs and TIC taking histopathology as a gold standard in oropharyngeal squamous cell carcinoma (OPSCC) biopsies.
Methods: A total of 122 biopsies of OPSCC were received for FS analysis at the Histopathology Department of the University College of Medicine and Dentistry Lahore, Pakistan. Touch imprints were taken from the surgical margins of the same biopsies and FSs were cut and stained with rapid Hematoxylin and Eosin stain following standard protocols. Paraffin-embedded histopathology sections were later made from the same tissues for comparison and diagnostic accuracy studies.
Results: Comparison of FS with histopathology showed a sensitivity of 60%, specificity of 100%, positive predictive value (PPV) of 100%, negative predictive value (NPV) of 95%, and overall diagnostic accuracy of 95%. On comparison of TIC with histopathology, sensitivity was 86.6%, specificity was 100%, PPV was 100%, NPV was 98.2%, and accuracy was 98.3%.
Conclusion: In comparison to histopathology, TIC and FS examination have promising utility as well as certain limitations for the evaluation of the margin status of OPSCC specimens. Thus, with careful consideration, these techniques may provide valuable insights for clinical practice in local settings with both time and cost-effective approaches.
Keywords: Frozen section, histopathology, touch imprint, diagnostic accuracy, specimens, surgical margins, oropharyngeal squamous cell carcinoma.
Pubmed Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica. 2024; 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153
Web Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. https://biomedicapk.com/articles/online_first/1153 [Access: September 01, 2024]. doi:10.24911/BioMedica/5-1153
AMA (American Medical Association) Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica. 2024; 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153
Vancouver/ICMJE Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica. (2024), [cited September 01, 2024]; 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153
Harvard Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar (2024) Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica, 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153
Chicago Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. "Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas." 25 (2024), 95-99. doi:10.24911/BioMedica/5-1153
MLA (The Modern Language Association) Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. "Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas." 25.June 2024 (2024), 95-99. Print. doi:10.24911/BioMedica/5-1153
APA (American Psychological Association) Style
Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar (2024) Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. , 25 (June 2024), 95-99. doi:10.24911/BioMedica/5-1153