BioMedica |

Original Article

Volume: 40 | Issue: 2 | Published: Jun 25, 2024 | Pages: 95 - 99 | DOI: 10.24911/BioMedica/5-1153

Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas

Authors: Hamna Salahuddin , Sadia Sharif , Shizra Kaleemi , Kanwal Babar , Abeer Zaheer , Sadia Anwar

Article Info

Authors

Hamna Salahuddin

University College of Medicine and Dentistry, Ameer-ud-din Medical College

Sadia Sharif

University College of Medicine and Dentistry

Shizra Kaleemi

Al Aleem Medical College

Kanwal Babar

Chughtai Lab, Lahore, Pakistan

Abeer Zaheer

Rahbar Medical & Dental College

Sadia Anwar

Ameeruddin Medical College

Publication History

Received: April 01, 2024

Revised: June 07, 2024

Accepted: June 20, 2024

Published: June 25, 2024

Abstract

Background and Objective: In surgical procedures, accurate intraoperative diagnosis is essential for guiding surgical management. Two common techniques employed for rapid intraoperative diagnosis are touch imprint cytology (TIC) and frozen section (FS). However, their diagnostic accuracy as compared to histopathology remains under debate. This study therefore aims to evaluate the diagnostic precision of FSs and TIC taking histopathology as a gold standard in oropharyngeal squamous cell carcinoma (OPSCC) biopsies.

Methods: A total of 122 biopsies of OPSCC were received for FS analysis at the Histopathology Department of the University College of Medicine and Dentistry Lahore, Pakistan. Touch imprints were taken from the surgical margins of the same biopsies and FSs were cut and stained with rapid Hematoxylin and Eosin stain following standard protocols. Paraffin-embedded histopathology sections were later made from the same tissues for comparison and diagnostic accuracy studies.

Results: Comparison of FS with histopathology showed a sensitivity of 60%, specificity of 100%, positive predictive value (PPV) of 100%, negative predictive value (NPV) of 95%, and overall diagnostic accuracy of 95%. On comparison of TIC with histopathology, sensitivity was 86.6%, specificity was 100%, PPV was 100%, NPV was 98.2%, and accuracy was 98.3%.

Conclusion: In comparison to histopathology, TIC and FS examination have promising utility as well as certain limitations for the evaluation of the margin status of OPSCC specimens. Thus, with careful consideration, these techniques may provide valuable insights for clinical practice in local settings with both time and cost-effective approaches.

Keywords: Frozen section, histopathology, touch imprint, diagnostic accuracy, specimens, surgical margins, oropharyngeal squamous cell carcinoma.

Pubmed Style

Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica. 2024; 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153

Web Style

Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. https://biomedicapk.com/articles/online_first/1153 [Access: September 13, 2024]. doi:10.24911/BioMedica/5-1153

AMA (American Medical Association) Style

Vancouver/ICMJE Style

Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica. (2024), [cited September 13, 2024]; 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153

Harvard Style

Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar (2024) Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. BioMedica, 25 (June 2024): 95-99. doi:10.24911/BioMedica/5-1153

Chicago Style

Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar. "Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas." 25 (2024), 95-99. doi:10.24911/BioMedica/5-1153

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Hamna Salahuddin, Sadia Sharif, Shizra Kaleemi, Kanwal Babar, Abeer Zaheer, Sadia Anwar (2024) Diagnostic accuracy of intraoperative touch imprint cytology and frozen sections for the evaluation of margin status and diagnosis of oro-pharyngeal squamous cell carcinomas. , 25 (June 2024), 95-99. doi:10.24911/BioMedica/5-1153