Original Article
VOLUME: 36 | ISSUE: 2 | Jun 30, 2020 | PAGE: (118 - 125) | DOI: 10.24911/BioMedica/5-124
A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery
Authors: Kawther Aabed , Nadine Moubayed , Abrar Motlak , Al-Anoud Al-Rasheed , Ameerah Al-Otaibi , Aisha Al-Asmri , Hanadi Abu-Dusha
Article Info
Authors
Kawther Aabed
Biology Department, College of Science Princess Nourah Bint Abdul Rahman University Riyadh, Saudi Arabia.
Nadine Moubayed
Biology Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
Abrar Motlak
Biology Department, College of Science, Princess Nourah Bint Abdul Rahman University, Riyadh, Saudi Arabia.
Al-Anoud Al-Rasheed
Biology Department, College of Science, Princess Nourah Bint Abdul Rahman University, Riyadh, Saudi Arabia.
Ameerah Al-Otaibi
Biology Department, College of Science, Princess Nourah Bint Abdul Rahman University, Riyadh, Saudi Arabia.
Aisha Al-Asmri
Biology Department, College of Science, Princess Nourah Bint Abdul Rahman University, Riyadh, Saudi Arabia.
Hanadi Abu-Dusha
Biology Department, College of Science, Princess Nourah Bint Abdul Rahman University, Riyadh, Saudi Arabia.
Publication History
Received: March 07, 2020
Revised: June 09, 2020
Accepted: June 15, 2020
Published: June 30, 2020
Abstract
Background and Objective: Human gut harbors a diverse community of more than 100 trillion microbial cells that play an important role in human metabolism, physiology, immune function, and nutrition. Similarly, disruptions to the composition of this population can be linked with gastrointestinal conditions,such as obesity and inflammatory bowel disease. The aim of the present study was to compare the microbial community characteristics among obese, normal-weight and post-gastric bariatric surgerypatients.
Methods: Fifteen healthy adults, who were classified into (1) normal-weight (n=5) (2) obese (n=5) according to their Body mass index (BMI) and (3) post-bariatric surgery (n=5) groups. Gut microbiota from fecal samples were profiled by the streak plate method and theconstituent populationswere identified by biochemical analysis (Vitek2). Finally, specific bacterial strains were identified via molecular techniques, such as Polymerase chain reaction (PCR). Moreover, the antimicrobial sensitivity of E. coli strains isolated from healthy adults was evaluated to determine the pathogenic strains.
Results: The findings revealed that gut microbiota diversity increased following bariatric surgery, whereby84.6% of increased bacteria belonged to Proteobacteria, especially Escherichia coli (member of Gammaproteobacteria). The main bacterial groups in individuals that underwent bariatric surgery were Gemella morbillorum, Citrobacter freundii, Serratia odorifera, Proteus mirabilis, Enterococcus gallinarum, Klebsiella pneumonia, Enterobacter cloacae complex, Pseudomonas aeruginosa and Escherichia coli. The main bacterial groups in the normal-weight group were Citrobacter freundii, Serratia marcescens, Proteus
mirabilis and Escherichia coli. Finally, in obese individuals, Enterobacter cloacae complex, Klebsiella pneumonia, Enterococcus gallinarum and Escherichia coli were the main bacterial groups.Conclusion: These results indicate that inducing changes in the gut microbiota may be a relevant therapeutic approach for obesity and other metabolic defects.
Keywords: Human, Gut microbiota, Obesity, Normal-weight, Bariatric surgery, Adults
Pubmed Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha. A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery. BioMedica. 2020; 30 (June 2020): 118-125. doi:10.24911/BioMedica/5-124
Web Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha. A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery. https://biomedicapk.com/articles/online_first/124 [Access: May 08, 2024]. doi:10.24911/BioMedica/5-124
AMA (American Medical Association) Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha. A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery. BioMedica. 2020; 30 (June 2020): 118-125. doi:10.24911/BioMedica/5-124
Vancouver/ICMJE Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha. A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery. BioMedica. (2020), [cited May 08, 2024]; 30 (June 2020): 118-125. doi:10.24911/BioMedica/5-124
Harvard Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha (2020) A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery. BioMedica, 30 (June 2020): 118-125. doi:10.24911/BioMedica/5-124
Chicago Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha. "A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery." 30 (2020), 118-125. doi:10.24911/BioMedica/5-124
MLA (The Modern Language Association) Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha. "A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery." 30.June 2020 (2020), 118-125. Print. doi:10.24911/BioMedica/5-124
APA (American Psychological Association) Style
Kawther Aabed, Nadine Moubayed, Abrar Motlak, Al-Anoud Al-Rasheed, Ameerah Al-Otaibi, Aisha Al-Asmri, Hanadi Abu-Dusha (2020) A Comparison of Human Gut Microbiota and Analysis of Antimicrobial Resistance of E. coli Isolates in Saudi Adults Undergoing Bariatric Surgery. , 30 (June 2020), 118-125. doi:10.24911/BioMedica/5-124