Original Article
VOLUME: 36 | ISSUE: 4 | Dec 31, 2020 | PAGE: (362 - 366) | DOI: 10.24911/BioMedica/5-80
Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species
Authors: Dr. Majid Rauf Ahmad , Dr. Iffat Javed , Dr. Sohaila Mushtaq , Dr. Rubeena Hafeez , Dr. Kanwal Hassan Cheema
Article Info
Authors
Dr. Majid Rauf Ahmad
Avicenna Medical College, Lahore-Pakistan.
Dr. Iffat Javed
Amina Inayat Medical College, Lahore-Pakistan.
Dr. Sohaila Mushtaq
Azra Naheed Medical College, Lahore-Pakistan.
Dr. Rubeena Hafeez
Avicenna Medical College, Lahore-Pakistan.
Dr. Kanwal Hassan Cheema
CMH Medical College, Lahore-Pakistan.
Publication History
Received: October 22, 2020
Revised: December 02, 2020
Accepted: December 14, 2020
Published: December 31, 2020
Abstract
Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment is started. Although direct microscopy is routinely performed but culture of dermatophytes is the gold standard. However, it takes about 4 weeks for species identification on primary media. Our aim was to compare dermatophyte test medium (DTM) as a screening medium for the isolation of dermatophytes in comparison with sabouraud dextrose agar (SDA).
Methods: It was a comparative study carried out at the Department of Microbiology of Post Graduate Medical Institute, Lahore over a period of nine months. Samples were collected from one hundred patients with clinically suspected dermatophytoses after taking informed written consent. The samples were examined microscopically and then inoculated on two types of culture media, one Sabouraud dextrose agar (SDA) with added chloramphenicol, gentacin and cycloheximide and other dermatophyte test medium (DTM) with added chlortetracycline, gentacin and cyclohexamide.
Results: Fungal growth was observed in fifty-six samples on culture. Out of the fifty-six positive on cultures, nineteen were that of dermatophytes. Out of n = 100 patients, ten were positive on SDA while n = 14 dermatophyte species were able to grow on DTM. A significantly higher positivity (P ³ 0.05) for isolating dermatophytes was observed by DTM as compared to SDA. DTM was able to isolate (71%) of the dermatophytes in first 10 days. Isolation rate of dermatophyte species was higher (73.68%) on DTM as compared to SDA which was 52.6%.
Conclusion: Authors recommend the use of dermatophyte test medium for the primary isolation and identification of dermatophyte species to be more effective and time saving.
Keywords: Dermatophytes, Sabouraud’s dextrose agar, Dermatophyte test medium
Pubmed Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema. Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species. BioMedica. 2020; 31 (December 2020): 362-366. doi:10.24911/BioMedica/5-80
Web Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema. Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species. https://biomedicapk.com/articles/online_first/80 [Access: April 28, 2024]. doi:10.24911/BioMedica/5-80
AMA (American Medical Association) Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema. Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species. BioMedica. 2020; 31 (December 2020): 362-366. doi:10.24911/BioMedica/5-80
Vancouver/ICMJE Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema. Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species. BioMedica. (2020), [cited April 28, 2024]; 31 (December 2020): 362-366. doi:10.24911/BioMedica/5-80
Harvard Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema (2020) Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species. BioMedica, 31 (December 2020): 362-366. doi:10.24911/BioMedica/5-80
Chicago Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema. "Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species." 31 (2020), 362-366. doi:10.24911/BioMedica/5-80
MLA (The Modern Language Association) Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema. "Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species." 31.December 2020 (2020), 362-366. Print. doi:10.24911/BioMedica/5-80
APA (American Psychological Association) Style
Dr. Majid Rauf Ahmad, Dr. Iffat Javed, Dr. Sohaila Mushtaq, Dr. Rubeena Hafeez, Dr. Kanwal Hassan Cheema (2020) Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species. , 31 (December 2020), 362-366. doi:10.24911/BioMedica/5-80