BioMedica |

Original Article

VOLUME: 35 | ISSUE: 1 | Mar 21, 2019 | PAGE: (24 - 27) | DOI: 10.51441.BioMedica.5-533

Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore

Authors: Khawaja A , Arshad F , Asif M , Ahmad S , Yunus N , Hameed A

Article Info

Authors

Khawaja A

Department of Pathology, Rahbar Medical & Dental College, Lahore-Pakistan.

Arshad F

Department of Pathology, Rahbar Medical & Dental College, Lahore-Pakistan.

Asif M

Department of Pathology, Rahbar Medical & Dental College, Lahore-Pakistan.

Ahmad S

Government Teaching Hospital, Shahdra, Lahore-Pakistan,

Yunus N

Fatima Memorial Medical & Dental College, Lahore-Pakistan.

Hameed A

Gujranwala Medical College, Gujranwala-Pakistan.

Publication History

Received: November 17, 2018

Revised: February 06, 2019

Accepted: March 02, 2019

Published: March 21, 2019

Abstract

Background and Objective: The versatility of Staphylococcus aureus has been transformed as “Methicillin resistant Staphylococcus aureus”. Among phenotypic methods for detection of MRSA, feasible technique, i.e., cefoxitin disc diffusion test and/or oxacillin disc diffusion test can significantly contribute to-wards reliable detection. The objective of the study is to determine methicillin resistance in Staphylococcus aureus by disc diffusion methods; and to evaluate their accuracy with mecA gene PCR for MRSA detection.
Methods: A total of 750 staphylococcus aureus isolates were screened with oxacillin disk (1 g) and cefoxitin disk (30 g) by Kirby-Bauer method using CLSI guideline (2016); to get 105 continuous, nonrepetitive clinical isolates of MRSA. All the methicillin resistant staphylococcus aureus isolates were further amplified by polymerase chain reaction for mecA gene detection.
Results: All the 105 MRSA isolates were resistant by both oxacillin and cefoxitin disk diffusion tests. On PCR amplification, out of 105 MRSA (oxacillin resistant) isolates, 83 (79.04%) isolates were positive for mecA gene. Among 105 MRSA (cefoxitin resistant) isolates, 89 (84.76%) were mecA gene positive, by PCR amplification. The sensitivity (96.73%) and diagnostic accuracy (94.28%) of cefoxitin disc diffusion method was higher than oxacillin disc diffusion technique, with reference to PCR as a gold standard.
Conclusion: Cefoxitin disc diffusion method can be employed reliably for detection of mecA gene in MR-SA isolates in settings with limited resources, where molecular methods are not available. This can efficiently reduce the misdiagnosis and dissemination of resistant strains.

Keywords: Methicillin resistant Staphylococcus aureus, Oxacillin, Cefoxitin, PCR.

Pubmed Style

Khawaja A, Arshad F, Asif M, Ahmad S, Yunus N, Hameed A. Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore. BioMedica. 2019; 21 (March 2019): 24-27. doi:10.51441.BioMedica.5-533

Web Style

Khawaja A, Arshad F, Asif M, Ahmad S, Yunus N, Hameed A. Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore. https://biomedicapk.com/articles/online_first/533 [Access: May 04, 2024]. doi:10.51441.BioMedica.5-533

AMA (American Medical Association) Style

Vancouver/ICMJE Style

Khawaja A, Arshad F, Asif M, Ahmad S, Yunus N, Hameed A. Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore. BioMedica. (2019), [cited May 04, 2024]; 21 (March 2019): 24-27. doi:10.51441.BioMedica.5-533

Harvard Style

Khawaja A, Arshad F, Asif M, Ahmad S, Yunus N, Hameed A (2019) Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore. BioMedica, 21 (March 2019): 24-27. doi:10.51441.BioMedica.5-533

Chicago Style

Khawaja A, Arshad F, Asif M, Ahmad S, Yunus N, Hameed A. "Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore." 21 (2019), 24-27. doi:10.51441.BioMedica.5-533

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Khawaja A, Arshad F, Asif M, Ahmad S, Yunus N, Hameed A (2019) Molecular Evaluation of Methicillin Resistant Staphylococcus Aureus Isolates at a Tertiary Care Hospital in Lahore. , 21 (March 2019), 24-27. doi:10.51441.BioMedica.5-533