Original Article
VOLUME: 37 | ISSUE: 1 | Mar 31, 2021 | PAGE: (17 - 23) | DOI: 10.51441/BioMedica/5-165
Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population
Authors: Sabahat Fatima , Zeenat Usman , Saqib Mehmood , Shagufta Khaliq
Article Info
Authors
Sabahat Fatima
Assistant Professor of Biochemistry Gujranwala Medical College, Gujranwala, Pakistan.
Zeenat Usman
Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.
Saqib Mehmood
Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.
Shagufta Khaliq
Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.
Publication History
Received: October 21, 2020
Revised: January 08, 2021
Accepted: March 11, 2021
Published: March 31, 2021
Abstract
Background and Objective: Premature ovarian failure is a worldwide concern effecting 1% of females of reproductive age. The objective of the present study was to analyze the role of inhibin alpha (INHα) gene mutation (769G>A) in patients with premature ovarian failure (POF) in the local population.
Methods: This case-control association studywas conducted in Department of Gynecology, Jinnah Hospital and The Children’s Hospital and Institute of Child Health from July 2015-July 2016. A total of n = 100 were recruited for this study and divided into two gropus females with equal number (n = 50)of patients andnormal controls of reproductive age (14 – 40 years). The screening of theINHα for 769G>A variation in exon 2 was done through DNA sequencing.
Results: A higher frequency of the major allele G was seen in both the patients (99%) and the controls (87%) while comparing to minor allele A (1% in patients and 13% in controls). None of the patients was found to be homozygous (AA = 0%) for allele A, whereas, four of the controls were homozygous (AA = 8%). The frequency of the minor A allele in controls was found to be statistically significant (P-value = 0.002).
Conclusion: An association of decreased risk of POFwith A allele of the 769G>A variant rather than increasing the risk of development of ovarian failure.
Keywords: Ovarian physiology, Inhibin alpha (INHα) gene, Hypothalamic- pituitary-ovarian axis, Premature ovarian failure (POF).
Pubmed Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica. 2021; 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165
Web Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. https://biomedicapk.com/articles/online_first/165 [Access: April 24, 2024]. doi:10.51441/BioMedica/5-165
AMA (American Medical Association) Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica. 2021; 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165
Vancouver/ICMJE Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica. (2021), [cited April 24, 2024]; 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165
Harvard Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq (2021) Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica, 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165
Chicago Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. "Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population." 31 (2021), 17-23. doi:10.51441/BioMedica/5-165
MLA (The Modern Language Association) Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. "Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population." 31.March 2021 (2021), 17-23. Print. doi:10.51441/BioMedica/5-165
APA (American Psychological Association) Style
Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq (2021) Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. , 31 (March 2021), 17-23. doi:10.51441/BioMedica/5-165