Original Article

Volume 37, Issue 3 Page (1 - 13)

https://doi.org/10.51441.BioMedica.5-499

Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan


Authors: Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman


Authors

Faryal Husnain

Resident Pathologist, Department of Chemical Pathology and Immunology, Chughtai Institute of Pathology, Lahore-Pakistan.

Muhammad Dilawar Khan

Professor and HOD, Department of Chemical Pathology and Immunology, Chughtai Institute of Pathology, Lahore-Pakistan.

Omar Rasheed Chughtai

Director Operations and Assistant Professor, Department of Histopathology, Chughtai Institute of Pathology, Lahore-Pakistan.

Akhtar Sohail Chughtai

CEO and Professor Histopathology, Chughtai Institute of Pathology, Lahore-Pakistan.

Shakeel Ashraf

Technical Supervisor, Department of Chemical Pathology and Immunology, Chughtai Institute of Pathology, Lahore-Pakistan.

Muhammad Usman

Biosafety Officer, Department of Chemical Pathology and Immunology, Chughtai Institute of Pathology, Lahore-Pakistan.

Publication History

Received: September 28, 2021

Accepted:

Published: October 07, 2021


Abstract


Background and Objective: Chitinase-3-like-1 Protein (CHI3L1) is an upcoming biomarker for the diagnosis of liver fibrosis. The reference intervals for CHI3L1 have not been established in the Pakistani population. Thus, this study aimed to determine the reference intervals in our population and to determine cut-off value for diagnosis of hepatic fibrosis

Methods: It was a cross-sectional study. A total of 408 participants (202 Healthy and 206 diagnosed liver fibrosis cases) were recruited. Serum CHI3L1 level was measured on CHI3L1 kits (Proprium Biotech Co. Ltd) by manual Enzyme Linked Immunosorbant Assay (ELISA).  The reference interval was estimated by percentile and working normal method.

Results: The distribution of CHI3L1 values showed no remarkable variation with gender and age. The 95% reference interval of chitinase 3 like 1 protein was 12.80ng/ml to 81.80ng/ml in healthy Pakistani subjects and the cut-off for the diagnosis was 102.12 ng/ ml in hepatic fibrosis cases.

Conclusion: The reference interval in healthy adults and the cut-off for the diagnosis of hepatic fibrosis of serum chitinase-3-like-1 protein is determined in a selective adult Pakistani population. This will be a useful reference  for further local and international studies.

 


Keywords: Chitinase 3 like Protein 1, Cirrhosis, Hepatic Fibrosis, Reference interval, Non-Alcoholic Fatty Liver Disease (NAFLD), Non-Alcoholic Steatohepatitis (NASH), Hepatocellular Carcinoma.



Pubmed Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. biomedica. 2021; 07 (October 2021): 86-91. doi:10.51441.BioMedica.5-499

Web Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. https://biomedicapk.com/10.51441/BioMedica/5-499 [Access: October 21, 2021]. doi:10.51441.BioMedica.5-499

AMA (American Medical Association) Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. biomedica. 2021; 07 (October 2021): 86-91. doi:10.51441.BioMedica.5-499

Vancouver/ICMJE Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. biomedica. (2021), [cited October 21, 2021]; 07 (October 2021): 86-91. doi:10.51441.BioMedica.5-499

Harvard Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman (2021) Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. biomedica, 07 (October 2021): 86-91. doi:10.51441.BioMedica.5-499

Turabian Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. 2021. Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. Biomedica, 07 (October 2021): 86-91. doi:10.51441.BioMedica.5-499

Chicago Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. "Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan." Biomedica 07 (2021), 86-91. doi:10.51441.BioMedica.5-499

MLA (The Modern Language Association) Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman. "Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan." Biomedica 07.October 2021 (2021), 86-91. Print. doi:10.51441.BioMedica.5-499

APA (American Psychological Association) Style

Faryal Husnain, Muhammad Dilawar Khan, Omar Rasheed Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf, Muhammad Usman (2021) Chitinase-3-like-Protein 1: Reference Interval of Healthy Population and its Diagnostic Value for Liver Fibrosis in Pakistan. Biomedica, 07 (October 2021), 86-91. doi:10.51441.BioMedica.5-499