Original Article

Volume 37, Issue 3 Page (1 - 8)

https://doi.org/10.51441.BioMedica.5-512

Differential Expression of Markers of Oxidative Stress and Apoptosis  in Relation to Serum Ferritin Levels in  Patients with Pre-eclampsia


Authors: Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin


Authors

Jianying Yan

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University-China.

Jie Dong

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China

Xiaoqian Lin

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China

Lichun Chen

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China

Zhuanji Fang

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China.

Qing Han

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China.

Qinjian Zhang

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China.

Lingling Jiang

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China.

Xia Xu

Fujian Provincial Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou-China.

Xu Lin

Key Lab of Ministry of Edu for Gastrointestinal Cancer, School of Basic Med Sci, Fujian Medical University, Fuzhou-China.

Publication History

Received: October 06, 2021

Accepted:

Published: October 06, 2021


Abstract


Background and Objective: Pre-eclampsia (PE) is a hypertensive gestational disease appearing during second trimester of pregnancy. Free 
radicals are released by the placenta in this condition that may cause oxidative damage. This study was designed to determine the serum ferritin (SF) levels in maternal blood, fetal umbilical cord blood and placenta and the changes associated with oxidative stress as well as cell 
apoptosis to understand the pathogenesis of PE.
Methods: This cross-sectional analytical study recruited 60 pregnant females with severe PE and assigned into early and late onset PE  groups. Another n = 60 cases of normal pregnant females with similar gestational weeks were selected in the control group. Maternal serum and fetal umbilical cord blood ferritin levels were determined by automatic biochemical immunoassay system. Reverse transcription  real-time fluorescence, Western blot and colorimetry were used to determine mRNA expression levels of ferritin and ferritin heavy chain, relative expression of ferritin and superoxide dismutase, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels, respectively.
Results: Mean age was 30.89 ± 5.65 and 31.79 ± 5.06 years in early and late onset PE groups respectively. Serum uric acid and creatinine levels of both PE groups were significantly higher than the normal pregnant females. SF levels were also higher in the participants of PE groups. However, the mRNA and ferritin protein levels in placental tissue were significantly lower in PE groups while comparing to controls. The cleaved caspase-3 protein, GSH-Px and MDA levels were significantly higher in both PE groups (p < 0.05). 
Conclusion: The alterations in factors related to oxidative stress and cell apoptosis in placental tissue may be helpful to understand the pathogenesis of PE and may provide potential biomarkers for the diagnosis of PE.


Keywords: Ferritin, pre-eclampsia, oxidative stress, apoptosis, pregnancy, biomarkers.



Pubmed Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. biomedica. 2021; 06 (October 2021): 86-91. doi:10.51441.BioMedica.5-512

Web Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. https://biomedicapk.com/10.51441/BioMedica/5-512 [Access: October 21, 2021]. doi:10.51441.BioMedica.5-512

AMA (American Medical Association) Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. biomedica. 2021; 06 (October 2021): 86-91. doi:10.51441.BioMedica.5-512

Vancouver/ICMJE Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. biomedica. (2021), [cited October 21, 2021]; 06 (October 2021): 86-91. doi:10.51441.BioMedica.5-512

Harvard Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin (2021) Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. biomedica, 06 (October 2021): 86-91. doi:10.51441.BioMedica.5-512

Turabian Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. 2021. Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. Biomedica, 06 (October 2021): 86-91. doi:10.51441.BioMedica.5-512

Chicago Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. "Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia." Biomedica 06 (2021), 86-91. doi:10.51441.BioMedica.5-512

MLA (The Modern Language Association) Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin. "Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia." Biomedica 06.October 2021 (2021), 86-91. Print. doi:10.51441.BioMedica.5-512

APA (American Psychological Association) Style

Jianying Yan, Jie Dong, Xiaoqian Lin, Lichun Chen, Zhuanji Fang, Qing Han, Qinjian Zhang, Lingling Jiang, Xia Xu, Xu Lin (2021) Differential Expression of Markers of Oxidative Stress and Apoptosis&nbsp; in Relation to Serum Ferritin Levels in&nbsp; Patients with Pre-eclampsia. Biomedica, 06 (October 2021), 86-91. doi:10.51441.BioMedica.5-512