Review Article
VOLUME: 36 | ISSUE: 2S | Jun 24, 2020 | PAGE: (115 - 120) | DOI: 10.24911/BioMedica//BioMedica/5-451
RT-PCR for COVID-19: Diagnosis Made Easy
Authors: Osheen Sajjad , Aiman Shahzad , Saqib Mahmood
Article Info
Authors
Osheen Sajjad
Institute of Biomedical and Allied Health Sciences, University of Health Sciences, Lahore– Pakistan.
Aiman Shahzad
Institute of Biomedical and Allied Health Sciences, University of Health Sciences, Lahore– Pakistan.
Saqib Mahmood
Head, Institute of Biomedical and Allied Health Sciences, University of Health Sciences, Lahore– Pakistan.
Publication History
Received: May 18, 2020
Revised: May 25, 2020
Accepted: June 18, 2020
Published: June 24, 2020
Abstract
Coronavirus disease COVID-19, caused by Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV2), is highly contagious and has been a pandemic since March 2020. The SARS-CoV-2 is an enveloped, single-stranded, positive-sense RNA viruswhich spreadsthrough air droplets by sneezing and coughing from affected person. The diagnosis of the COVID-19 remains a challenge to the scientists since the genome of the SARS-CoV-2 was novel and varying. Various studies have reported the validated procedures for sampling and the detection method of SARS-CoV-2. This mini-review provides a brief introduction of the SARS-CoV-2 features and the current knowledge for the recommended COVID19 detection methods including sampling procedures and real time SARS-CoV-2 genome detection.
Keywords: RNA extraction, Real time-PCR, Diagnosis, COVID-19
