Original Article

Volume: 37 | Issue: 1 | Published: Mar 31, 2021 | Pages: 17 - 23 | DOI: 10.51441/BioMedica/5-165

Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population


Authors: Sabahat Fatima , Zeenat Usman , Saqib Mehmood , Shagufta Khaliq


Article Info

Authors

Sabahat Fatima

Assistant Professor of Biochemistry Gujranwala Medical College, Gujranwala, Pakistan.

Zeenat Usman

Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.

Saqib Mehmood

Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.

Shagufta Khaliq

Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.

Publication History

Received: October 21, 2020

Revised: January 08, 2021

Accepted: March 11, 2021

Published: March 31, 2021


Abstract


Background and Objective:  Premature ovarian failure is a worldwide concern effecting 1% of females of reproductive age. The objective of the present study was to analyze the role of inhibin alpha (INHα) gene mutation (769G>A) in patients with premature ovarian failure (POF) in the local population.

Methods:  This case-control association studywas conducted in Department of Gynecology, Jinnah Hospital and The Children’s Hospital and Institute of Child Health  from July 2015-July 2016. A total of n = 100  were recruited for this study and divided into two gropus females with equal number (n = 50)of patients andnormal controls of reproductive age (14 – 40 years). The screening of theINHα for 769G>A variation in exon 2 was done through DNA sequencing.

Results:  A higher frequency of the major allele G was seen in both the patients (99%) and the controls (87%) while comparing to minor allele A (1% in patients and 13% in controls). None of the patients was found to be homozygous (AA = 0%) for allele A, whereas, four of the controls were homozygous (AA = 8%). The frequency of the minor A allele in controls was found to be statistically significant (P-value = 0.002).

Conclusion:  An association of decreased risk of POFwith A allele of the 769G>A variant rather than increasing the risk of development of ovarian failure.


Keywords: Ovarian physiology, Inhibin alpha (INHα) gene, Hypothalamic- pituitary-ovarian axis, Premature ovarian failure (POF).


Pubmed Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica. 2021; 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165

Web Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. https://biomedicapk.com/articles/165 [Access: December 03, 2024]. doi:10.51441/BioMedica/5-165

AMA (American Medical Association) Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica. 2021; 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165

Vancouver/ICMJE Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica. (2021), [cited December 03, 2024]; 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165

Harvard Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq (2021) Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. BioMedica, 31 (March 2021): 17-23. doi:10.51441/BioMedica/5-165

Chicago Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. "Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population." 31 (2021), 17-23. doi:10.51441/BioMedica/5-165

MLA (The Modern Language Association) Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq. "Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population." 31.March 2021 (2021), 17-23. Print. doi:10.51441/BioMedica/5-165

APA (American Psychological Association) Style

Sabahat Fatima, Zeenat Usman, Saqib Mehmood, Shagufta Khaliq (2021) Genetic Analysis of Inhibin Alpha (INHα) Mutation (769G>A) in Patients with Premature Ovarian Failure in a Local Population. , 31 (March 2021), 17-23. doi:10.51441/BioMedica/5-165